Coding

Part:BBa_K5301013:Design

Designed by: xuan zhang   Group: iGEM24_BNU-China   (2024-09-23)


spNW15


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 496
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 496
    Illegal NheI site found at 64
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 496
    Illegal BglII site found at 552
    Illegal BglII site found at 924
    Illegal BamHI site found at 97
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 496
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 496
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
    Illegal AgeI site found at 1027
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Realize self circularization of MSP and reference the ApoA-1 sequence to enable efficient binding with phospholipids.


Source

It's genetically engineered based on Apolipoprotein A-1(ApoA-1)

References